Gel Electrophoresis Reagents and Buffers
LAMDA BIOTECH INC DNA SafeStain
DNA SafeStain is a new and safe nucleic acid stain for the visualization of doublestranded DNA, single-stranded DNA, and RNA in agarose gels. The dyes are developed to replace toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. DNA SafeStain is noncarcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests. DNA SafeStain emits green fluorescence when bound to DNA. It has two excitation wavelength peaks when bound to nucleic acid, at 290nm and 490nm. The emission wavelength for the DNA SafeStain is 530nm.
ABclonal Technology NDUFA10 Rabbit pAb
The protein encoded by this gene is a component of 42 kDa complex I, the first enzyme complex in the electron transport chain of mitochondria. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. A mutation in this gene was found in an individual with Leigh syndrome.
ABclonal Technology HOXA13 Rabbit mAb
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Expansion of a polyalanine tract in the encoded protein can cause hand-foot-uterus syndrome, also known as hand-foot-genital syndrome.
ABclonal Technology ADAMTS2 Rabbit pAb
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature procollagen N-proteinase. This proteinase excises the N-propeptide of the fibrillar procollagens types I-III and type V. Mutations in this gene cause Ehlers-Danlos syndrome type VIIC, a recessively inherited connective-tissue disorder. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
ABclonal Technology MOV10 Rabbit mAb
Enables 5-3 RNA helicase activity and RNA binding activity. Involved in defense response to virus, negative regulation of transposition, RNA-mediated, and posttranscriptional regulation of gene expression. Located in P-body and cytosol. Implicated in hypertension.
ABclonal Technology LY75 Rabbit pAb
Predicted to enable signaling receptor activity. Predicted to be involved in immune response and inflammatory response. Located in extracellular exosome.
ABclonal Technology SMARCA1 Rabbit pAb
This gene encodes a member of the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription. The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells. Alternative splicing results in multiple transcript variants.
ABclonal Technology TRPM4 Rabbit pAb
The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes, thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration, but this channel does not transport calcium.
Sigma Aldrich Fine Chemicals Biosciences ECLTM Prime Western Blotti
Amersham(TM) ECL(TM) Prime is a highly sensitive reagent characterized by stable signal emission allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. The high intensity of the emitted signals means that proteins can be detected using highly diluted primary and secondary antibodies contributing to low background and reducing consumption of reagents.Amersham(TM) ECL(TM) Prime consolidates and builds on the benefits of Amersham(TM) ECL(TM) Plus and Amersham(TM) ECL(TM) Advance to deliver a detection system that is sensitive stable precisely quantitative across a wide dynamic range of protein levels and conservative in its consumption of expensive antibody reagents.
National Diagnostics AccuGel 29:1 (30%), 1 Liter
AccuGel 29:1 is a stabilized, ready-to-use solution of 30% (w/v) acrylamide : bisacrylamide(29:1). AccuGel 29:1 has zero acrylic acid content, eliminating the fixed charges that cause band streaking. Additionally, oxidation products such as aldehydes have been removed by a selective adsorption process. With AccuGel 29:1, you can trust that your results will be consistent from one electrophoretic run to the next. It is certified RNase and DNase Free.
ABclonal Technology CRYAB Rabbit mAb
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins consist of two gene products, alpha-A and alpha-B, which are part of the small heat shock protein (HSP20) family. Induced by heat shock, they act as molecular chaperones by holding proteins in large soluble aggregates, rather than renaturing and releasing them like true chaperones. These aggregates consist of 30-40 subunits with a 3:1 ratio of alpha-A to alpha-B. Alpha crystallins also have autokinase activity and contribute to intracellular architecture. Alpha-A is mainly expressed in the lens, while alpha-B is widely expressed in many tissues. Elevated alpha-B expression is linked to neurological diseases, and a missense mutation is associated with desmin-related myopathy. Alternative splicing produces multiple isoforms.
ABclonal Technology KIF3A Rabbit mAb
Enables protein phosphatase binding activity, small GTPase binding activity, and spectrin binding activity. Involved in protein localization to cell junction and protein transport. Located in centriole and centrosome. Part of kinesin II complex. Colocalizes with spindle microtubule.
ABclonal Technology LEF1 Rabbit pAb
This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.