STE Buffer 1X Solution (Sodium Chloride, TRIS, EDTA) is a versatile laboratory buffer used primarily in DNA extraction and purification protocols. The 500 mL solution is ready-to-use and contains sodium chloride to maintain osmotic balance, TRIS to stabilize the pH, and EDTA to chelate divalent cations, which helps in the protection of nucleic acids. This buffer is essential for ensuring that DNA remains intact during extraction processes, preventing degradation from nucleases. It is widely used in molecular biology applications, including PCR, gel electrophoresis, and other DNA-based assays where maintaining DNA integrity is crucial.